Gene Expression Technology

양장본 Hardcover

Goeddel, 저자(글)

Academic Press · 1990년 05월 28일

책 소개

The methods presented in this volume will enable the reader to design effective strategies for the expression of cloned genes and cDNAs and will prove useful in solving the majority of expression problems one is likely to encounter.
"The purpose of this volume is to give recombinant DNA researchers the most up-to-date knowledge about heterologous gene expression in four commonly employed organisms: Escherichia coli, Bacillus subtilis, yeast, and mammalian cells....This synthesis of theory and practice has, in general, been skillfully done, making this volume most worthwhile for students and researchers in this area. Expert coverage in each of the four organisms ensures that the knowledge and advice given in each area is first rate....This volume should prove invaluable to investigators with a molecular biology background who need to express a given gene, but are not particularly experienced in this area. Many of the contributors have been able to effectively communicate years worth of experience and unpublished observations in what often was a black magic science. This volume clearly shows that things have changed and that the methodology is now in the hands of the skilled non-expert." --AMERICAN JOURNAL OF PHYSIOLOGY "The volume is very successful...I strongly recommend this book both to novices who are embarking on a gene expression project for the first time and to frustrated veterans who are seeking the latest 'tips' about how to improve the expression of their favourite proteins." --TRENDS IN GENETICS "This volume presents an extremely valuable collection of methods for expression of cloned genes...This volume fills an important void in the scientific literature. It is designed to be an integrated treatment of expression systems rather than a collection of relevant papers. In most respects it accomplishes that goal admirably and it provides an invaluable resource that should be present in any laboratory interested in manipulating gene expression." --ANALYTICAL BIOCHEMISTRY "This work is essential for the experienced worker or novice who having cloned his gene wishes to express it." --BIOTECHNOLOGY & APPLIED BIOCHEMISTRY
Introduction: D.V. Goeddel, Systems for Heterologous Gene Expression. Expression in Escherichia coli: L. Gold, Expression of Heterologous Proteins in Escherichia coli. Vectors: P. Balbas and F. Bolivar, Design and Construction of Expression Plasmid Vectors in Escherichia coli. Promoters: J.D. Gralla, Promoter Recognition and mRNA Initiation by Escherichia coli Eo - 70. D.G. Yansura and D.J. Henner, Use of Escherichia coli trp Promoter for Direct Expression of Proteins. F.W. Studier, A.H. Rosenberg, J.J. Dunn, and J.W. Dubendorff, Use of T7 RNA Polymerase to Direct Expression of Cloned Genes. Translation: L. Gold and G.D. Stormo, High-Level Translation Initiation. B.E. Schoner, R.M. Belagaje, and R.G. Schoner, Enhanced Translational Efficiency with Two-Cistron Expression System. H.A. de Boer and A.S. Hui, Sequences within Ribosome Binding Site Affecting Messenger RNA Translatability and Method to Direct Ribosomes to Single Messenger RNA Species. P.O. Olins and S.H. Rangwala, Vector for Enhanced Translation of Foreign Genes in Escherichia coli. Proteases: S. Gottesman, Minimizing Proteolysis in Escherichia coli: Genetic Solutions. Gene Fusions: M. Uhlen and T. Moks, Gene Fusions for Purpose of Expression: An Introduction. B. Nilsson and L. Abrahmsen, Fusions to Staphylococcal Protein A. D.G. Yansura, Expression as trpE Fusion. Secretion: J.A. Stader and T.J. Silhavy, Engineering Escherichia coli to Secrete Heterologous Gene Products. Protein Refolding: T. Kohno, D.F. Carmichael, A. Sommer, and R.C. Thompson, Refolding of Recombinant Proteins. Expression in Bacillus subtilis: D.J. Henner, Expression of Heterologous Genes in Bacillus subtilis. Intracellular Production: S.F.J. Le Grice, Regulated Promoter for High-Level Expression of Heterologous Genes in Bacillus subtilis. Secretion: V. Nagarajan, System for Secretion of Heterologous Proteins in Bacillus subtilis. Inducible Expression of Regulatory Proteins: D.J. Henner, Inducible Expression of Regulatory Genes in Bacillus subtilis. Expression in Yeast: S.D. Emr, Heterologous Gene Expression in Yeast. Plasmid Vectors: A.B. Rose and J.R. Broach, Propagation and Expression of Cloned Genes in Yeast: 2-micro;Circle-Based Vectors. T.Stearns, H. Ma, and D. Botstein, Manipulating Yeast Genome Using Plasmid Vectors. Inducible Expression Cassettes: L.M. Mylin, K.J. Hofmann, L.D. Schultz, and J.E. Hopper, Regulated GAL4 Expression Cassette Providing Controllable and High-Level Output from High-Copy Galactose Promoters in Yeast. V.L. Price, W.E. Taylor, W. Clevenger, M. Worthington, and E.T. Young, Expression of Heterologous Proteins in Saccharomyces cerevisiae Using ADH2 Promoter. T. Etcheverry, Induced Expression Using Yeast Coppe

작가정보

저자(글) Goeddel,

목차

• Introduction
  Systems for Heterologous Gene Expression
  Expression in Escherichia coli
  Expression of Heterologous Proteins in Escherichia coli
  Vectors
  Design and Construction of Expression Plasmid Vectors in Escherichia coli
  Promoters
  Promoter Recognition and mRNA Initiation by Escherichia coli Eo - 70
  Use of Escherichia coli trp Promoter for Direct Expression of Proteins
  Use of T7 RNA Polymerase to Direct Expression of Cloned Genes
  Translation
  High-Level Translation Initiation
  Enhanced Translational Efficiency with Two-Cistron Expression System
  Sequences within Ribosome Binding Site Affecting Messenger RNA Translatability and Method to Direct Ribosomes to Single Messenger RNA Species
  Vector for Enhanced Translation of Foreign Genes in Escherichia coli
  Proteases
  Minimizing Proteolysis in Escherichia coli: Genetic Solutions
  Gene Fusions
  Gene Fusions for Purpose of Expression: An Introduction
  Fusions to Staphylococcal Protein A
  Expression as trpE Fusion
  Secretion
  Engineering Escherichia coli to Secrete Heterologous Gene Products
  Protein Refolding
  Refolding of Recombinant Proteins
  Expression in Bacillus subtilis
  Expression of Heterologous Genes in Bacillus subtilis
  Intracellular Production
  Regulated Promoter for High-Level Expression of Heterologous Genes in Bacillus subtilis
  Secretion
  System for Secretion of Heterologous Proteins in Bacillus subtilis
  Inducible Expression of Regulatory Proteins
  Inducible Expression of Regulatory Genes in Bacillus subtilis
  Expression in Yeast
  Heterologous Gene Expression in Yeast
  Plasmid Vectors
  Propagation and Expression of Cloned Genes in Yeast: 2-micro;Circle-Based Vectors
  Manipulating Yeast Genome Using Plasmid Vectors
  Inducible Expression Cassettes
  Regulated GAL4 Expression Cassette Providing Controllable and High-Level Output from High-Copy Galactose Promoters in Yeast
  Expression of Heterologous Proteins in Saccharomyces cerevisiae Using ADH2 Promoter
  Induced Expression Using Yeast Coppe
  Table of Contents provided by Publisher. All Rights Reserved.

기본정보

ISBN	9780121820862 ( 0121820866 )
발행(출시)일자	1990년 05월 28일
쪽수	681쪽
크기	160 * 235 * 52 mm / 1193 g
총권수	1권
언어	영어